Tolerance of the Rat for N,N'-Diphenyl-P-Phenylenediamine

Abstract

Inclusion of DPPD in a vitamin E-low diet fed to female rats through growth and reproduction resulted in varying degrees of reproductive failure. Toxicity was encountered during growth at a level of 0.5% but not at 0.1% or below. Rats receiving 0.1% DPPD exhibited uterine and vaginal hemorrhages at parturition accompanied by anorexia, anemia and emaciation. Concentrations of 0.025%, 0.01% and 0.005% resulted in a high incidence of stillbirth. No adverse effects were encountered from feeding 0.0006% DPPD in the diet. Data show that DPPD elicits symptoms of toxicity in the rat at concentrations below those used in commercial mixed feeds.