Genetic studies of D-alanine-dehydrogenase-less mutants ofEscherichia coliK12
- 1 October 1981
- journal article
- research article
- Published by Hindawi Limited in Genetics Research
- Vol. 38 (2), 197-208
- https://doi.org/10.1017/s0016672300020528
Abstract
Genetic analysis of 12 mutants of Escherichia coli K12 defective in D-alanine dehydrogenase showed that alnA and dad are alternative names for the same locus. dad was shown to be a single gene which codes for a protein of 55000–60000 mol. wt. Study of thermosensitive mutants of dad indicated that its product is a structural component of D-alanine dehydrogenase. The regulatory gene alnR was shown to be involved in positive control of dad expression. This was concluded from (i) the absence of constitutive strains among Dad+ revertants of alnR– mutations, (ii) the trans dominance of alnR+ to alnR–, and (iii) the failure to isolate fully constitutive strains by any means. To obtain a uniform nomenclature it is proposed to re-name dad as dadB and alnR as dadQ.This publication has 24 references indexed in Scilit:
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