Kinetics of ATP‐ and noradrenaline‐mediated sympathetic neuromuscular transmission in rat tail artery

Abstract

Electrophysiological, electrochemical and mechanical recordings were employed to study the kinetics of the release and clearance of adenosine 5'-triphosphate (ATP) and noradrenaline (NA) as sympathetic co-transmitters and of the neurogenic and non-neurogenic contractions in rat isolated tail artery. The life-time of ATP and NA released by a single pulse or 10 pulses at 50 Hz was brief (< 100 ms, or < 3 s, respectively); the neurogenic contractile responses occurred largely after the transmitters had been removed from the extracellular space. The ATP-induced neurogenic contractile responses to a single pulse or 10 pulses at 50 Hz were similar in time-course to the responses to direct muscle stimulation at low voltage; both seemed to be caused by activation of nifedipine-sensitive voltage-gated L-type Ca2+ channels. The alpha 1- and alpha 2-adrenoceptor-mediated components of the NA-induced neurogenic contractile response to 10 pulses at 50 Hz were more delayed and prolonged and determined by properties of the post-receptor mechanisms. The per pulse release of both ATP and NA faded rapidly during long high-frequency trains. So did the ATP level at the receptors and the ATP-induced neurogenic contraction. The NA levels and the contractile responses induced via alpha 1- and alpha 2-adrenoceptors were much better maintained during ongoing stimulation at 20 Hz but relaxed rapidly afterwards, suggesting that nerve activity suppressed, and cessation of nerve activity reactivated NA clearance.