Localization of thyroglobulin antigenicity in rat thyroid sections using antibodies labled with peroxidase or (125)I-radioiodine
Open Access
- 1 September 1977
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 74 (3), 992-1015
- https://doi.org/10.1083/jcb.74.3.992
Abstract
In the hope of localizing thyroglobulin within focullar cells of the thyroid gland, antibodies raised against rat thyroglobulin were labeled with the enzyme horseradish peroxidase or with (125)I-radioiodine. Sections of rat thyroids fixed in glutaraldehyde and embedded in glycol methacrylate or Araldite were placed in contact with the labeled antibodies. The sites of antibody binding were detected by diaminobenzidine staining in the case of peroxidase labeling, and radioautography in the case of 125(I) labeling.This publication has 53 references indexed in Scilit:
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