High‐ and Low‐Affinity Binding of [3H]Imipramine in Rat Hypothalamus
- 1 November 1986
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 47 (5), 1609-1613
- https://doi.org/10.1111/j.1471-4159.1986.tb00802.x
Abstract
In the rat hypothalamus [3H]imipramine binding is inhibited by tricyclic and nontricyclic antidepressant drugs in a complex manner, with biphasic curves and Hill coefficients 3H]-imipramine binding sites as the [3H]imipramine concentration was raised. In the absence of sodium ions, IC50 values for the inhibition by tricyclic and nontricyclic antidepressants were increased by approximately 1,000-fold, and the inhibition curves became classically monophasic with Hill coefficients close to 1.0. These data are interpreted as suggesting that [3H]imipramine binds to two independent sites in the rat hypothalamus. One site is sodium-dependent with a high affinity for the drugs tested; the other is sodium-independent and has a low affinity for these drugs.Keywords
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