Isolation and characterization of a 7 S RNP particle from mature Xenopus laevis oocytes

Abstract
Mature oocytes of Xenopus laevis contain a 7 S RNP particle consisting of two components, ribosomal 5 S RNA and a protein of M r ≈45 000. The structure of the free 5 S rRNA and the 7 S RNP complex has been studied by diethylpyrocarbonate modification of adenines. A74, A77, A90, A100 and A103 of the 5 S rRNA are protected upon association of the protein.