Ultratrace Enrichment of Tyrosine Phosphorylated Peptides on an Imprinted Polymer
- 9 February 2011
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 83 (5), 1862-1865
- https://doi.org/10.1021/ac103086v
Abstract
Novel molecularly imprinted polymers (MIPs) designed to bind the side chain of phosphotyrosine can be used as artificial receptors for affinity-based enrichment of proteolytic peptides. In comparison with general enrichment methods for phosphorylated peptides such as TiO2-based methods, the pTyr-imprinted polymers offered high selectivity for pTyr-containing peptides down to the low fmol level. This suggests MIPs as a new tool for affinity-based proteomics.Keywords
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