Inactivation of thioredoxin by sulfite ions
- 30 July 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 268 (1), 146-148
- https://doi.org/10.1016/0014-5793(90)80994-t
Abstract
Oxidized thioredoxin undergoes sulfitolysis of its single disulfide bond at low concentrations of sulfite ions and protein and in the absence of denaturing agents. The reaction, which has an optimum at pH 8, was studied using [35S]sulfite and E. coli thioredoxin as model. The product, thioredoxin-S-sulfonate, has a half-life of several hours in solution. It is unable to activate chloroplast NADP malate dehydrogenase. Thioredoxin sulfitolysis may therefore be a physiologically important factor in mediating the phytotoxic effects ofsulfur dioxide in plants.Keywords
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