Storage proteins are present in the hemolymph from larvae and adults of the Colorado potato beetle
- 1 January 1992
- journal article
- research article
- Published by Wiley in Archives of Insect Biochemistry and Physiology
- Vol. 20 (2), 119-133
- https://doi.org/10.1002/arch.940200204
Abstract
The protein composition of larval and adult hemolymph from the Colorado potato beetle, Leptinotarsa decemlineata, was investigated and some abundant, high molecular weight proteins were identified and characterized. Diapause protein 1, which occurs in the hemolymph of last instar larvae and short-day adults, appeared to be a storage protein. This protein dissociated into two bands due to the high pH used in nondenaturing gels. Its quaternary structure was established by chemical crosslinking. It appeared to be a hexamer. Diapause protein 1 is composed of ≈82,000 subunits. The amino acid composition and N-terminal sequence of this protein has been determined. Specific antibodies against diapause protein 1 have been developed. Topical application of 1 μg pyriproxyfen, a juvenile hormone analog, to last instar larvae and short-day adults suppressed the appearance of this protein in the hemolymph. Pyriproxyfen prematurely induced vitellogenin, when applied to last instar larvae. A larval specific protein was also identified in the hemolymph. Its temporary appearance in the hemolymph of last instar larvae, its subunit composition (Mr ≈82,000) and its suppression by pyriproxyfen suggests that this protein is a storage protein as well.Keywords
This publication has 23 references indexed in Scilit:
- Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetiiCellular Microbiology, 2007
- The Function and Evolution of Insect Storage HexamersAnnual Review of Entomology, 1991
- Thirty‐five years of diapause research with the Colorado potato beetleEntomologia Experimentalis et Applicata, 1990
- Effects of a juvenile hormone analogue on the eggs, post‐embryonic development, metamorphosis and diapause induction of the Colorado potato beetle, Leptinotarsa decemlineataEntomologia Experimentalis et Applicata, 1989
- Molecular characteristics of lipophorin, the juvenile hormone-binding protein in the hemolymph of the Colorado potato beetleArchives of Insect Biochemistry and Physiology, 1987
- High‐molecular‐weight serum proteins from Locusta migratoria: identification of a protein specifically binding juvenile hormone IIIPhysiological Entomology, 1986
- Vitellogenin and Vitellin in InsectsAnnual Review of Entomology, 1979
- Synthesis of vitellogenins and diapause proteins by the fat body of Leptinotarsa, as a function of photoperiodPhysiological Entomology, 1978
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The relation between haemolymph proteins and vitellogenesis in the Colorado beetle, Leptinotarsa decemlineataJournal of Insect Physiology, 1970