Cyclic AMP receptor protein interacts with lactose operator DNA
- 24 January 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (2), 277-292
- https://doi.org/10.1093/nar/9.2.277
Abstract
The wildtype lac promoter-operator region of E.coli was reinvestigated for cyclic AMP receptor protein (CRP) binding sites using the “footprinting’ method. Two distinct interaction sites were found, one in the −60 base pair region, which has previously been described, and a second one covering the lac operator. These two sites are similar in extent, orientation and the characteristic pattern of CRP-induced DNaseI enhanced cutting sites.Keywords
This publication has 30 references indexed in Scilit:
- Nucleotide sequence of the rightward operator of phage lambda.Proceedings of the National Academy of Sciences, 1975
- Genetic Regulation: The Lac Control RegionScience, 1975
- Activation of Transcription at Specific Promoters by GlycerolJournal of Biological Chemistry, 1974
- The Nucleotide Sequence of the lac OperatorProceedings of the National Academy of Sciences, 1973
- lac repressor-operator interaction: V. Characterization of super- and pseudo-wild-type repressorsJournal of Molecular Biology, 1972
- Mechanism of Initiation of Repression of In Vitro Transcription of the Lac Operon of Escherichia coliProceedings of the National Academy of Sciences, 1971
- The nature of Lactose operator constitutive mutationsJournal of Molecular Biology, 1971
- Cyclic AMP Receptor Protein of E. coli: Its Role in the Synthesis of Inducible EnzymesProceedings of the National Academy of Sciences, 1970
- The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel ElectrophoresisJournal of Biological Chemistry, 1969
- The promoter-operator region of the Lac operon of Escherichia coliJournal of Molecular Biology, 1968