The Freezing Point Depression of Bull Semen and Its Relation to the Diluter Problem

Abstract

From cryoscopic data it was found that fresh, normal bull semen has an osmotic pressure approaching that of cattle blood. The osmotic pressure of bull semen increased with length of storage in the excurrent ducts of the male or outside the body. The semen of two low fertility bulls depressed the freezing point more than did that of normal, fertile bulls. Heating sodium citrate solutions increases their osmotic pressure. For heated solutions, 2.9 grams of Na₃C₆H₅O₇ ⋅ 2H₂O per 100 ml. of water distilled in glass is isotonic with blood. Copyright © . .