A Specific Population of Gonadotrophs Purified from Immature Female Rat Pituitary
- 19 November 1976
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 194 (4267), 848-851
- https://doi.org/10.1126/science.790569
Abstract
When dispersed pituitary cells from 14-day-old female rats were sedimented in a bovine serum albumin gradient, a fraction was isolated which consisted of almost 90 percent of large cells that stained purple in the periodic acid-Schiff (PAS) reaction. Immunostaining indicated that over 85 percent of these PAS-purple cells were gonadotrophs containing both follicle stimulating and luteinizing hormone. Reproducible cell cultures could be obtained on poly-L-lysine coated dishes. As early as the second day in culture, the secretion of both follicle stimulating and luteinizing hormone was highly stimulated by luteinizing hormone-releasing hormone. The effect of FSH was as marked as that on LH. The data suggest that the isolated gonadotrophs are a specific functional subtype.This publication has 14 references indexed in Scilit:
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