A Comparison of Paramyxoviruses by Immunoprecipitation

Abstract

Using the technique of immune precipitation of [35S]methionine-labeled infected [African green monkey kidney Vero] cell polypeptides, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography, it was shown that simian virus 5 and a closely related isolate are both antigenically related to human parainfluenza virus type 2. Limited cross-reactions were also demonstrated between parainfluenza virus types 1 and 3 by this method, and the apparent MW of the major structural components of human parainfluenza virus types 2 and 3 were deduced.