The construction in vitro of transducing derivatives of phage lambda
- 1 January 1976
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 146 (2), 199-207
- https://doi.org/10.1007/bf00268089
Abstract
Methods are described for the construction of plaque-forming, transducing derivatives of phage lambda, using appropriate receptor genomes and fragments of DNA generated by the restriction enzymes endo R.EcoRI and endo R.HindIII. The general properties of the transducing derivatives are described and discussed. Plaque-forming phages carrying the E. coli trp, his, cysB, thyA, supD, supE, supF, hsd, tna and lig genes have been isolated.Keywords
This publication has 62 references indexed in Scilit:
- Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coliJournal of Molecular Biology, 1975
- Prophage lambda at unusual chromosomal locations: III. The components of the secondary attachment sitesJournal of Molecular Biology, 1975
- Interaction of the recombination pathways of bacteriophage λ and its host Escherichia coli K12: Effects on exonuclease V activityJournal of Molecular Biology, 1972
- Prophage lambda at unusual chromosomal locations: I. Location of the secondary attachment sites and the properties of the lysogensJournal of Molecular Biology, 1972
- Integrative recombination function of bacteriophage λ: Evidence for a site-specific recombination enzymeJournal of Molecular Biology, 1968
- Integration-negative mutants of bacteriophage lambdaJournal of Molecular Biology, 1968
- Host specificity of DNA produced by Escherichia coli: Bacterial mutations affecting the restriction and modification of DNAJournal of Molecular Biology, 1966
- A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology, 1961
- The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage λdgJournal of Molecular Biology, 1960
- TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATEProceedings of the National Academy of Sciences, 1958