Dodecyl sulphate/polyacrylamide-gel electrophoresis at low pH values and low temperatures
- 1 September 1979
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 181 (3), 759-761
- https://doi.org/10.1042/bj1810759
Abstract
A simple method is described for dodecyl sulphate/polyacrylamide-gel electrophoresis of pH- and temperature-labile biological intermediates. The method is based on a catalyst system that works at temperatures of 2–4 degrees C and pH values of 2–4 and an appropriate buffer system containing Li+ or Tris [CH2OH–C(CH2OH)2–NH3+] instead of Na+. This system does not lead to the precipitation of 1% dodecyl sulphate.Keywords
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