The duplicated human alpha globin genes lie close together in cellular DNA.
- 1 December 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (12), 5950-5954
- https://doi.org/10.1073/pnas.75.12.5950
Abstract
The organization of .alpha.-globin genes in normal human DNA was examined by restriction endonuclease mapping. .alpha.-Globin-specific fragments in endonuclease digests of total cell DNA were identified after electrophoresis by hybridization with [32P]cDNA following the blotting procedure of Southern (1975). The data provide direct evidence for the duplication of .alpha.-genes and further indicate that these loci are closely linked within a single restriction fragment. The HindIII sites (codons 90/91) of these duplicated genes lie approximately 3.7 kilobases apart in the physical map proposed for this region. This organization of .alpha.-genes can be altered in DNA of individuals with .alpha.-thalassemia.This publication has 24 references indexed in Scilit:
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