Reorganization of the Cytoskeleton by Interferon in MSV-Transformed Cells*

Abstract

.beta. and .gamma. interferons [IFN] enhanced the organization of microfilaments, intermediate filaments and fibronectin in a murine sarcoma virus (MSV)-transformed [mouse] cell line. Both IFN increased the number of intermediate-type junctions. Sodium butyrate also acted in a similar way and, when associated with IFN, an additive effect was observed. In a cell line cultured for many passages in the presence of .beta. interferon (MSV-IF+), the cytoskeletal network and the extra-cellular matrix were highly developed and reminiscent of a normal cell. These results could explain some of the biological actions of IFN, such as increased cell adhesion to the substratum and decreased cell motility.