Quantitation of Baclofen in Tablets Using High-Performance Liquid Chromatography

Abstract

A reverse phase high-performance liquid chromatography method has been developed to quantify baclofen in tablets. The method is accurate and precise with a percent relative standard deviation of 0.52 based on 5 readings. The recovery from the synthetic mixtures was quantitative. The results were in excellent agreement with the USP-NF colorimetric method. The method can be used to test the content uniformity of the tablets. Samples which were treated with either sulfuric acid or sodium hydroxide and boiled for 10 minutes did not show new peaks in the chromatogram. Baclofen appears to be a very stable compound.