Activation of the MAP kinase homologue RK requires the phosphorylation of Thr‐180 and Tyr‐182 and both residues are phosphorylated in chemically stressed KB cells
Open Access
- 8 May 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 364 (2), 223-228
- https://doi.org/10.1016/0014-5793(95)00346-b
Abstract
A MAP kinase homologue, termed the reactivating kinase (RK), lies in a signalling pathway which mediates cellular responses to stress. Here we demonstrate that the stress‐induced activation of the RK in human KB cells is accompanied by the phosphorylation of Thr‐180 and Tyr‐182, and that the phosphorylation of both residues is required for the activation of this enzyme.Keywords
This publication has 23 references indexed in Scilit:
- SB 203580 is a specific inhibitor of a MAP kinase homologue which is stimulated by cellular stresses and interleukin‐1FEBS Letters, 1995
- Inactivation of p42 MAP kinase by protein phosphatase 2A and a protein tyrosine phosphatase, but not CL100, in various cell linesCurrent Biology, 1995
- Specificity of receptor tyrosine kinase signaling: Transient versus sustained extracellular signal-regulated kinase activationCell, 1995
- Interleukin-1 activates a novel protein kinase cascade that results in the phosphorylation of hsp27Cell, 1994
- A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteinsCell, 1994
- A MAP Kinase Targeted by Endotoxin and Hyperosmolarity in Mammalian CellsScience, 1994
- MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivoCell, 1993
- An Osmosensing Signal Transduction Pathway in YeastScience, 1993
- Identification of MAPKAP kinase 2 as a major enzyme responsible for the phosphorylation of the small mammalian heat shock proteinsFEBS Letters, 1992
- Ordered phosphorylation of p42mapk by MAP kinase kinaseFEBS Letters, 1992