Dynamic histology of the antral epithelium in the mouse stomach: II. Ultrastructure and renewal of isthmal cells
- 1 March 1985
- journal article
- research article
- Published by Wiley in Journal of Anatomy
- Vol. 172 (3), 205-224
- https://doi.org/10.1002/aja.1001720304
Abstract
The isthmus of typical mucous units of the pyloric antrum was investigated in 3- to 4-month-old CD1 mice using light and electron microscopy as well as 3H-thymidine radioautography. On the average, the isthmus measured 25 μm in length and was composed of 36 isthmal cells and two enteroendocrine cells. Isthmal cells generally displayed features found in embryonic cells, such as many free ribosomes, scant organelles, and a large reticulated nucleolus, and were, therefore, at an immature stage of development. Isthmal cells could be devoid of secretory granules (“granule-free cells,” 2%) or contain a few small, spherical, PA-Schiffpositive, mucous granules in their apex. The granules in some of the cells had a variegated appearance and a diameter averaging 235 nm (“mottled granule cells,” 39%); in other cells, the granules had a large diameter, 278 nm, with a pale background and a dense core (“core granule cells,” 28%); while in still others they were homogeneously dark and measured 264 nm (“dense granule cells,” 12%). Finally, some cells included a mixture of core and dense granules (“mixed granule cells,” 14%). One hour after a single injection of 3H-thymidine, 37% of the isthmal cells were labeled. Each of the five isthmal cell types could acquire label and, therefore, divide. After one or more days of continuous 3H-thymidine infusion, all isthmal cells were labeled. Their turnover time was estimated to be 16.1 hr (t1/2 = 11.2 hr). The isthmus is thus composed of several cell types which are turning over rapidly. While all are relatively immature, the various types are thought to represent different developmental stages in the life history of an isthmal cell. A model devised on this basis proposes that the granule-free cells are stem cells, from which mottled granule cells are derived. These in turn evolve into either the dense granule cells of the upper isthmus or the core granule cells of the lower isthmus, or into the mixed granule cells (which are believed to develop eventually into dense granule cells or core granule cells). Maintenance of a steady state requires that the rapid production of isthmal cells be associated with rapid emigration; the dense granule cells presumably going to the pit and the core granule cells to the gland. The turnover of isthmal cells is accordingly described as following a “bidirectional pattern” of renewal.This publication has 29 references indexed in Scilit:
- Identification of Stem Cells in the Small Intestine of the MousePublished by Elsevier ,1976
- Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine I. Columnar cellJournal of Anatomy, 1974
- Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine II. Mucous cellsJournal of Anatomy, 1974
- Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine V. Unitarian theory of the origin of the four epithelial cell typesJournal of Anatomy, 1974
- Renewal of the epithelium in the descending colon of the mouse. I. Presence of three cell populations: Vacuolated‐columnar, mucous and argentaffinJournal of Anatomy, 1971
- Electron Microscopic Observations on Cell Proliferation and Differentiation in the Gastric Mucosa of the MouseArchivum histologicum japonicum, 1970
- Renewal of Paneth cells in the small intestine of the mouseJournal of Anatomy, 1969
- Dynamic Analysis of the Stomach EpitheliumArchivum histologicum japonicum, 1966
- IMPROVEMENTS IN THE COATING TECHNIQUE OF RADIOAUTOGRAPHYJournal of Histochemistry & Cytochemistry, 1962
- Ueber die schlauchförmigen Drüsen des Magendarmkanals und die Beziehungen ihres Epithels zu dem Oberflächenepithel der Schleimhaut Dritte MittheilungArchiv für Mikroskopische Anatomie, 1893