Selective control of cytosolic glutathione peroxidase and phospholipid hydroperoxide glutathione peroxidase mRNA stability by selenium supply
- 3 June 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 387 (2-3), 157-160
- https://doi.org/10.1016/0014-5793(96)00493-0
Abstract
Selenium depletion of H4 hepatoma cells reduced cytosolic glutathione peroxidase (cGSH-Px) mRNA abundance but had no effect on phospholipid hydroperoxide glutathione peroxidase (PHGSH-Px) mRNA abundance. Actinomycin D chase experiments showed that selenium depletion had no effect on the stability of PHGSH-Px mRNA but decreased the stability of cGSH-Px mRNA. In Se-replete cells puromycin decreased the stability of both cGSH-Px and PHGSH-Px mRNAs. The results suggest that when selenium supply is limiting PHGSH-Px mRNA translation is maintained more than that of cGSH-Px mRNA, and thus more cGSH-Px mRNA is released from polysomes and degraded.Keywords
This publication has 20 references indexed in Scilit:
- Type 3 lodothyronine deiodinase: cloning, in vitro expression, and functional analysis of the placental selenoenzyme.Journal of Clinical Investigation, 1995
- Selenium Regulation of Glutathione Peroxidase in Human Hepatoma Cell Line Hep3BArchives of Biochemistry and Biophysics, 1993
- Phospholipid Hydroperoxide Glutathione-Peroxidase: Full-Length Pig Blastocyst cDNA Sequence and Regulation by Selenium StatusBiochemical and Biophysical Research Communications, 1993
- Type I iodothyronine deiodinase is a selenocysteine-containing enzymeNature, 1991
- Phospholipid hydroperoxide glutathione peroxidase in various mouse organs during selenium deficiency and repletionBiochimica et Biophysica Acta (BBA) - General Subjects, 1990
- The effects of selenium depletion and repletion on the metabolism of thyroid hormones in the ratJournal of Inorganic Biochemistry, 1990
- Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the geneNucleic Acids Research, 1985
- Structure and variation of human ribosomal DNA: molecular analysis of cloned fragmentsGene, 1981
- Halogenated benzimidazole ribosidesBiochemical Pharmacology, 1978
- The phosphorylation of ribosomal protein S6 in baby hamster kidney fibroblastsBiochemical and Biophysical Research Communications, 1976