The binding of pyridoxal 5-phosphate to aspartate aminotransferase of pig heart
- 1 July 1963
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 88 (1), 172-175
- https://doi.org/10.1042/bj0880172
Abstract
Conditions are described under which aspartate aminotransferase can be dissociated into coenzyme and apoenzyme. Only the pyridoxamine form of the enzyme can be resolved. The resolution can be performed by incubation with several anions, of which phosphate is the most effective. The conditions under which phosphate is effective in resolving aspartate aminotransferase suggest the possibility that this phenomenon has physiological significance.Keywords
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