RADIOIMMUNOASSAY OF HUMAN GROWTH HORMONE USING THE DOUBLE ANTIBODY TECHNIQUE
- 1 April 1968
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 57 (4), 549-556
- https://doi.org/10.1530/acta.0.0570549
Abstract
The method of Ross et al. (1963) for the preparation of HGH yields a highly purified biologically active hormone preparation suitable for clinical purposes and, after one additional purification step (chromatography on Sephadex G-200 according to Hunter (1965)), for radioiodination. In the double antibody technique described, the labeled hormone can be used for radio-immunoassay without starch-gel or Sephadex purification. The method is simple and sensitive enough to permit the determination of HGH in serum.This publication has 10 references indexed in Scilit:
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