Involvement of protein dynamics in enzyme stability
- 23 September 2001
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 506 (3), 216-220
- https://doi.org/10.1016/s0014-5793(01)02917-9
Abstract
Dynamics of glucose oxidase immobilized and in solution were compared through their tryptophan fluorescence spectra, decay times and quenching by acrylamide. Energy barrier for thermal inactivation and melting temperature of both soluble and immobilized enzyme were also measured. Data show that the fluctuation amplitude is at the origin of protein instability.Keywords
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