UGA is read as tryptophan in Mycoplasma capricolum.
- 1 April 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (8), 2306-2309
- https://doi.org/10.1073/pnas.82.8.2306
Abstract
UGA is a nonsense or termination (opal) codon throughout prokaryotes and eukaryotes. However, mitochondria use not only UGG but also UGA as a tryptophan codon. Here, we show that UGA also codes for tryptophan in Mycoplasma capricolum, a wall-less bacterium having a genome only 20-25% the size of the Escherichia coli genome. This conclusion is based on the following evidence. First, the nucleotide sequence of the S3 and L16 ribosomal protein genes from M. capricolum includes UGA codons in the reading frames; they appear at positions corresponding to tryptophan in E. coli S3 and L16. Second, a tRNATrp gene and its product tRNA found in M. capricolum have the anticodon sequence 5' U-C-A 3', which can form a complementary base-pairing interaction with UGA.This publication has 24 references indexed in Scilit:
- A different genetic code in human mitochondriaNature, 1979
- The primary structure of protein S3 from the small ribosomal subunit of Escherichia coliFEBS Letters, 1979
- Use of the UGA terminator as a tryptophan codon in yeast mitochondria.Proceedings of the National Academy of Sciences, 1979
- Direct chemical method for sequencing RNA.Proceedings of the National Academy of Sciences, 1979
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- The primary structure of protein L16 located at the peptidyltransferase center of Escherichia coli ribosomesFEBS Letters, 1976
- A primer ribonucleic acid for initiation of in vitro Rous sarcarcoma virus deoxyribonucleic acid synthesis.Journal of Biological Chemistry, 1975
- The primary structure of tryptophan transfer ribonucleic acid from brewer's yeast: II. — Partial digestion with pancreatic ribonuclease and derivation of complete sequenceBiochimie, 1972
- Tryptophan transfer RNA as the UGA suppressorJournal of Molecular Biology, 1971
- Codon—anticodon pairing: The wobble hypothesisJournal of Molecular Biology, 1966