Immobilization can improve the stability of hybridoma antibody productivity in serum‐free media
- 5 December 1990
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 36 (10), 1049-1055
- https://doi.org/10.1002/bit.260361010
Abstract
Hybridoma cells (S3H5/γ2bA2) were cultivated in spinner flasks with 1% serum media and serum-free media. Monoclonal antibody productivity was maintained in 1% serum media. However, cells in serum-free media showed a decrease in antibody productivity, and it completely disappeared in IMDM-based low protein medium. This loss of antibody productivity was not observed when the cells were immobilized in alginate beads. In fact, immobilization enhanced the specific MAb productivity.This publication has 21 references indexed in Scilit:
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