Multi‐site phosphorylation of branched‐chain 2‐oxoacid dehydrogenase complex within mitochondria isolated from rat liver, kidney and heart
- 28 November 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 164 (1), 85-88
- https://doi.org/10.1016/0014-5793(83)80024-6
Abstract
The α subunit of the E1 component of branched-chain 2-oxoacid dehydrogenase complex becomes rapidly phosphorylated in rat liver, kidney and heart mitochondria incubated in the presence of succinate and [32P]phosphate. Peptide mapping of tryptic digests of the phosphorylated α subunit indicates that 3 distinct sites are phosphorylated, as has been reported previously by us for phosphorylation in vitro of highly purified complexKeywords
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