OXIDATION OF METHIONINE RESIDUES IN BOVINE GROWTH HORMONE BY CHLORAMINE-T
- 12 January 2009
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 16 (4), 299-305
- https://doi.org/10.1111/j.1399-3011.1980.tb02590.x
Abstract
The methionine residues in bovine growth hormone were chemically modified with chloramine-T at pH 7.4 using different chloramine-T methionine molar ratios. Methionine 4 was the most reactive followed in decreasing order by methionines 148, 123 and 178. With a 50-fold molar excess of chloramine-T over methionine almost full oxidation of all the methionine residues was achieved. The oxidation of the 4 residues of methionine does not change the growth promoting activity of the hormone in hypophysectomized rats.Keywords
This publication has 16 references indexed in Scilit:
- REACTION OF BOVINE AND EQUINE GROWTH HORMONES WITH TETRANITROMETHANEInternational Journal of Peptide and Protein Research, 2009
- ANTIBODIES AGAINST HAPTENS IN THE STUDY OF STRUCTURE AND BIOLOGICAL ACTIVITY OF GROWTH HORMONEInternational Journal of Peptide and Protein Research, 1979
- Selective oxidation of methionine residues in proteinsBiochemistry, 1975
- The primary structure of bovine growth hormoneFEBS Letters, 1973
- Primary Structure of Bovine Growth HormoneEuropean Journal of Biochemistry, 1973
- Reaction of bovine and ovine pituitary growth hormones with tetranitromethaneBiochemistry, 1973
- Carboxymethylation of methionine residues in bovine growth hormoneFEBS Letters, 1972
- The amino acid sequence of bovine growth hormoneFEBS Letters, 1971
- Bovine growth hormone: Evidence for two allelic formsBiochemical and Biophysical Research Communications, 1971
- G-Terminal sequence of amino acids in bovine growth hormoneBiochemical and Biophysical Research Communications, 1965