Examination of the Chrysophyceae flora in Otter Lake, British Columbia, by electron microscopy verified the occurrence of eight scale-bearing species belonging to the genera Epipyxis, Spiniferomonas, Chrysosphaerella, Mallomonas, and Mallomonopsis. Two of the species, Epipyxis kenaiensis and Mallomonopsis paxillata, are reported from Canada for the first time. This report also includes notes on the taxonomy and distribution of the eight species of Chrysophyceae and basic limnological data for Otter Lake.