Monoclonal Antibodies to Murine Immunoglobulin Isotypes
- 1 January 1983
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 2 (1), 49-54
- https://doi.org/10.1089/hyb.1983.2.49
Abstract
We have obtained cell lines producing monoclonal antibodies against μ, α, λ, and λ1 by fusing the mouse myeloma P3X63Ag8.653 with spleen cells from Lewis rats immunized with several λ1 bearing myeloma proteins. The anti-λ1 antibody is particularly useful. It was shown to be suitable for radioimmunoassays, for ELISAs when coupled to alkaline phosphatase, and for indirect fluorescence staining of the membrane as well as the cytoplasm.Keywords
This publication has 6 references indexed in Scilit:
- Use of Monoclonal Anti-mouse Immunoglobulin to Detect Mouse AntibodiesHybridoma, 1981
- A New Mouse Myeloma Cell Line that Has Lost Immunoglobulin Expression but Permits the Construction of Antibody-Secreting Hybrid Cell LinesThe Journal of Immunology, 1979
- Immunoglobulin production by lymphocyte hybridomasEuropean Journal of Immunology, 1978
- Antibodies to major histocompatibility antigens produced by hybrid cell linesNature, 1977
- Variant strain of rabbits lacking immunoglobulin κ polypeptide chainNature, 1977
- A simplified method for cyanogen bromide activation of agarose for affinity chromatographyAnalytical Biochemistry, 1974