Light-induced enzyme system leading to pigmentation
- 1 March 1979
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 21 (3), 443-459
- https://doi.org/10.1002/bit.260210307
Abstract
α‐Chymotrypsin was light sensitized by acylating with cis‐cinnamoyl ester, a substrate interconvertible to the trans form by ultraviolet (UV) light. The degree of acylation by this method was complete leaving no residual activity of the enzyme. Upon UV irradiation the inhibited enzyme regained about 70% of its original activity, thereby adding light‐sensitiveness to the proteolytic enzyme. In seeking a photographic application of the light‐sensitized enzyme, a pigmenting enzyme was incorporated with it. The coupled enzyme system was shown to exhibit a light signal in the form of dark pigment slurry.This publication has 11 references indexed in Scilit:
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