Histochemical Studies of Leukocytes from an Inflammatory Exudate

Abstract

Activity of alkaline phosphatases, acid phosphatases and esterases was studied histochemically in migrating leukocytes from "skin windows", using azo dye coupling techniques. Esterases were demonstrated both by the a-naphthyl acetate method and the naphthol AS-D chloroacetate method. The different types of migrating leukocytes presented different patterns of activity. Leukocytes from blood films were examined using the same methods, and a noticeable similarity was observed between the enzymatic pattern of exudative macrophages and blood monocytes. The experiments also showed that the two esterase methods do not demonstrate the same enzymes.