Physical Evidence for the Asembly of A and B Chains of Human Placental Collagen in a Single Triple Helix
Open Access
- 1 December 1978
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 92 (2), 563-567
- https://doi.org/10.1111/j.1432-1033.1978.tb12778.x
Abstract
Native collagen molecules containing A and B chains were isolated from pepsin-solubilised human chorionic and amniotic membrane extracts by fractional salt precipitation and DEAE-cellulose chromatography. They exhibited a circular dichroism spectrum, and a melting curve, characteristic for a triple-helical structure. Electron microscopical investigations of their segment-long-spacing crystallites revealed a molecule similar to those of the interstitial types I, II and III collagens. After denaturation, the A and B chains were separated by DEAE-cellulose chromatography and were consistently recovered in a ratio of 1:2. Renaturation experiments indicated that only the B chains are able to reform triple-helical molecules which are stable under conditions in vivo. The data support a molecular formula A(B)2 for the native collagen molecule.This publication has 18 references indexed in Scilit:
- Physicochemical characterization and molecular organization of the collagen A and B chainsBiochemistry, 1978
- Nature of the Collagenous Protein in a Tumor Basement MembraneEuropean Journal of Biochemistry, 1978
- Three new α-chains of collagen from a non-basement membrane sourceBiochemical and Biophysical Research Communications, 1978
- Biochemical characteristics and biological significance of the genetically-distinct collagensMolecular and Cellular Biochemistry, 1976
- Bovine renal glomerular basement membrane. Assessment of proteolysis during isolationBiochemical and Biophysical Research Communications, 1976
- Isolation of three collagenous components of probable basement membrane origin from several tissuesBiochemical and Biophysical Research Communications, 1976
- Comparative Electron‐Microscope Studies on the Collagens Extracted from Cartilage, Bone, and SkinEuropean Journal of Biochemistry, 1972
- Isolation of a collagen from basement membranes containing three identical α-chainsBiochemical and Biophysical Research Communications, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The Formation of Triple‐Helical Collagen Molecules from α1 or α2 Polypeptide ChainsEuropean Journal of Biochemistry, 1969