Stimulation and inhibition of anti-hapten responses in guinea pigs immunized with hybrid liposomes.
- 1 November 1975
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (11), 4582-4586
- https://doi.org/10.1073/pnas.72.11.4582
Abstract
Guinea pigs were immunized with liposomal model membranes containing phosphatidylethanolamine (PE) or glycerophosphorylethanolamine (GPE) derivatives in which the amino function was substituted with dinitrophenylaminocaproyl (Dnp-Cap) or mono(p-azobenzenearsonic acid)tyrosyl (ABA-Tyr) residues. Previous studies demonstrated that hapten-specific antibodies are elicited by Dnp-Cap-PE or ABA-Tyr-PE sensitized liposomes and that cell-mediated immunity is induced by ABA-Tyr-PE (but not Dnp-Cap-PE) sensitized liposomes. These liposomes differ from conventional immunogens in which haptens are covalently attached to immunogenic carriers. This investigation describes 2 new aspects of liposomal immunogenicity in animals immunized with hybrid liposomes containing Dnp-Cap-PE and ABA-Tyr-PE: stimulation of the anti-Dnp response by incorporation of increasing amounts of ABA-Tyr-PE and inhibition of anti-ABA antibody formation by incorporation of increasing amounts of Dnp-Cap-PE. The 2 phenomena are dependent on the presence of each determinant in the same lipid bilayer. Entrapment of the water-soluble deacylated derivative of ABA-Tyr-PE (i.e., ABA-Tyr-GPE) in the aqueous compartments of Dnp-Cap-PE sensitized liposomes does not enhance anti-Dnp antibody production. Entrapment of the non-amphipathic derivative of Dnp-Cap-PE (i.e., Dnp-Cap-GPE) within ABA-Tyr-PE sensitized liposomes does not suppress anti-ABA anti-body formation. Mixtures of Dnp-Cap-PE sensitized liposomes and ABA-Tyr-PE sensitized liposomes neither stimulated nor inhibited the anti-hapten responses. Preparation of hybrid liposomes with different N-substituted PE derivatives may provide an extremely convenient method for controlling hapten and/or immunologic carrier determinant density.This publication has 10 references indexed in Scilit:
- Immunogenicity of liposomal model membranes sensitized with mono(p-azobenzenearsonic acid)tyrosylphosphatidylethanolamine derivatives. Antibody formation and delayed hypersensitivity reactionBiochemistry, 1975
- Immune Response to Liposomal Model Membranes: Restricted IgM and IgG Anti-Dinitrophenyl Antibodies Produced in Guinea PigsThe Journal of Immunology, 1975
- Antibody formation in response to liposomal model membranes sensitized with N-substituted phosphatidylethanolamine derivativesBiochemistry, 1974
- Effect of immunoglobulin class and affinity on the initiation of complement-dependent damage to liposomal model membranes sensitized with dinitrophenylated phospholipidsBiochemistry, 1973
- Active vs. passive sensitization of liposomes toward antibody and complement by dinitrophenylated derivatives of phosphatidylethanolamineBiochemistry, 1972
- ANTIGEN RECOGNITION AND THE IMMUNE RESPONSEThe Journal of Experimental Medicine, 1972
- Antigen Recognition and the Immune Response: the Capacity of L-Tyrosine-Azobenzenearsonate to Serve as a Carrier for a Macromolecular HaptenThe Journal of Immunology, 1971
- Determination of Antibody-Hapten Equilibrium Constants by an Ammonium Sulfate Precipitation TechniqueThe Journal of Immunology, 1969
- COMPETITION OF HASTENSThe Journal of Experimental Medicine, 1966
- IMMUNOCHEMICAL STUDY OF ANTIGENIC SPECIFICITY IN DELAYED HYPERSENSITIVITYThe Journal of Experimental Medicine, 1966