Nucleotide sequence of ilvGEDA operon attenuator region of Escherichia coli.
- 1 April 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (4), 1823-1827
- https://doi.org/10.1073/pnas.77.4.1823
Abstract
The nucleotide sequence of the DNA thought to contain the control region for the ilvGEDA operon in E. coli was determined by the Maxam-Gilbert procedure. The sequence includes a region that, upon transcription, would yield a leader transcript specifying a peptide 32 residues long. This putative peptide would contain 4 leucine, 5 isoleucine and 6 valine residues. A model is proposed that correlates the multivalent control of the ilvGEDA operon with the extent to which this leader transcript is translated. In vitro transcription experiments yielded a transcript of about 183 nucleotides, compatible with the predictions of the model.This publication has 20 references indexed in Scilit:
- Nucleotide sequence of the promoter-operator region of the tryptophan operon of Escherichia coliJournal of Molecular Biology, 1978
- Transcription termination at the trp operon attenuators of Escherichia coli and Salmonella typhimurium: RNA secondary structure and regulation of termination.Proceedings of the National Academy of Sciences, 1977
- Determination of the nucleotide sequence of part of the regulatory region for the galactose operon from Escherichia coli.Journal of Biological Chemistry, 1977
- Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresisBiochemistry, 1975
- In vitro synthesis of the E3 immunity protein directed by Col E3 plasmid deoxyribonucleic acid.Journal of Biological Chemistry, 1975
- Stability of ribonucleic acid double-stranded helicesJournal of Molecular Biology, 1974
- Improved Estimation of Secondary Structure in Ribonucleic AcidsNature New Biology, 1973
- Isolation of transducing bacteriophages for the histidine and isoleucine-valine operons in Escherichia coli K-12.1972
- Oligonucleotide Sequence of Replicase Initiation Site in Qβ RNANature New Biology, 1972
- Polypeptide Chain Initiation: Nucleotide Sequences of the Three Ribosomal Binding Sites in Bacteriophage R17 RNANature, 1969