Isolated perfused rat lung preparation.

Abstract

A simple system is described for the perfusing of blood in a closed system through an isolated rat lung. The preparation appears to be functioning metabolically for at least 3 hr. Glucose was utilized as indicated by a fall in the glucose concentration in the circulating blood and an increase in the lactic acid concentration. The glycolysis appeared to be due to the metabolic activity of the lung tissue rather than the addition of some material by the lung to the perfusing blood which stimulated the metabolism of the erythrocytes.