Molecular Cloning of Single-stranded RNAs of Potato Leafroll Virus and Beet Western Yellows Virus
- 1 September 1988
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 69 (9), 2397-2402
- https://doi.org/10.1099/0022-1317-69-9-2397
Abstract
Complementary DNA libraries were synthesized using the genomic RNAs of potato leafroll virus (PLRV) and beet western yellows virus (BWYV), by random as well as oligo(dT) priming of polyadenylated RNA, and cloned into pBR322. Two restriction endonuclease maps were constructed, extending to 61 kbp and 5.5 kbp for the PLRV and BWYV genomes respectively. The 3'' ends of genomic RNAs were verified by sequence analysis of oligo(dT)-primed clones; 5''-terminal sequences of PLRV snd BWYV were not detected among the cDNA fragments mapped. Selected clones were used to demonstrate specific virus detection in total nucleic acid preparations of PLRV- or BWYV-infected Physalis floridana Rydb. One PLRV-derived clone showed cross-hybridization with purified BWYV RNA.This publication has 17 references indexed in Scilit:
- Studies on transformation of Escherichia coli with plasmidsJournal of Molecular Biology, 1983
- Evidence that Potato Leafroll Virus RNA is Positive-stranded, is Linked to a Small Protein and Does Not Contain PolyadenylateJournal of General Virology, 1982
- Purification and characterization of potato leafroll virusVirology, 1979
- Enzyme-assisted Purification of two Phloem-limited Plant Viruses: Tobacco Necrotic Dwarf and Potato LeafrollJournal of General Virology, 1979
- Isolation and Analysis of Double-Stranded RNA from Virus-Infected Plant and Fungal TissuePhytopathology®, 1979
- Spliced early mRNAs of simian virus 40Proceedings of the National Academy of Sciences, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.Proceedings of the National Academy of Sciences, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977