Agents affecting lipid metabolism. XXXVII. Separation of rat serum lipoproteins with dextran sulfate

Abstract

Dextran sulfate was used to separate rat serum lipoproteins into insoluble low-density and soluble high-density fractions. Lipid composition of the separated fractions was compared to that of lipoprotein fractions prepared by ultracentrifugation; data indicate that dextran sulfate precipitates lipoproteins with a density lower than 1.063.In rats treated with ethyl p-chlorophenoxyisobutyrate and estradiol-17β comparable, though not identical, changes in the composition of serum lipoproteins were observed with both methods of separation. The observed differences may reflect the fundamental difference in the basis of separation used by the methods. Data obtained with dextran sulfate may indicate changes in the protein moieties of lipoproteins.