dC-dG Alternating Oligonucleotides: Thermodynamic and Kinetic Aspects of the B-Z Transformation
- 1 February 1987
- journal article
- research article
- Published by Taylor & Francis in Journal of Biomolecular Structure and Dynamics
- Vol. 4 (4), 651-662
- https://doi.org/10.1080/07391102.1987.10507666
Abstract
The alternating cytosine-guanine oligodeoxyribonucleotides (dCdG)n, (dGdC)n, (dCdG)n, (dCdG)ndC (n=3,4), (dGdC)7 and dG(dCdG)3 have been studied by UV and CD spectroscopy at different temperatures and NaCl concentrations. The analysis of the melting data, assuming an all-or-none model, reveals that in the B-conformation the 5′G/C3′ stacking interactions are enthalpically favoured with respect to the 5′C/G3′ one. The CD investigation of the B-Z equilibrium shows that the Z-conformation is enthalpically stabilized, while the B-conformation is entropically favoured, in the range of NaCl concentration considered (1 to 5M). The kinetic data for the B-Z transformation, obtained with a salt-jump technique for the hexamer (dCdG)3, support a mechanism by which (he Watson-Crick hydrogen bonds are broken before the bases flip over separately and eventually stack, reforming the H-bonds, in the new helix.This publication has 17 references indexed in Scilit:
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