Contactless conductivity detection of selected organic ions in on‐chip electrophoresis
- 1 March 2004
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 25 (6), 903-908
- https://doi.org/10.1002/elps.200305732
Abstract
The detection of underivatized anionic sulfonates, carboxylates, amino acids, sugars, and artificial sweeteners, and of cationic dopamine, ephedrine, and metanephrine in microfabricated electrophoresis devices is demonstrated. This was achieved by high‐voltage contactless conductivity measurements with external electrodes. Poly(methyl methacrylate) chips with thin covers to enable sensitive contactless detection were used for most determinations but glass microchips had to be employed for amino acids and sugars. The plastic chips were found not be stable in the alkaline media required to render those two classes of species in the ionic form amenable for separation and detection. The reproducibility of peak area measurements was about 1% or better and the detection limits ranged between 1 and 30 μM for the different compounds examined.Keywords
This publication has 28 references indexed in Scilit:
- High‐voltage contactless conductivity detection of underivatized amino acids in capillary electrophoresisElectrophoresis, 2003
- High-voltage contactless conductivity-detection for lab-on-chip devices using external electrodes on the holderThe Analyst, 2003
- Separation of twenty underivatized essential amino acids by capillary zone electrophoresis with contactless conductivity detectionElectrophoresis, 2003
- On-Chip Contactless Four-Electrode Conductivity Detection for Capillary Electrophoresis DevicesAnalytical Chemistry, 2002
- Contact Conductivity Detection in Poly(methyl methacylate)-Based Microfluidic Devices for Analysis of Mono- and Polyanionic MoleculesAnalytical Chemistry, 2002
- Improved capacitively coupled conductivity detector for capillary electrophoresisThe Analyst, 2002
- Recent developments in detection methods for microfabricated analytical devicesLab on a Chip, 2001
- Dual photometric-contactless conductometric detector for capillary electrophoresisAnalytica Chimica Acta, 2001
- Capillary Electrophoretic Determination of Different Classes of Organic Ions by Potentiometric Detection with Coated-Wire Ion-Selective ElectrodesAnalytical Chemistry, 1998
- Contactless Conductivity Detection for Capillary ElectrophoresisAnalytical Chemistry, 1998