Functional Purification and Enzymic Characterization of the RNA-dependent DNA Polymerase of Human Immunodeficiency Virus
- 1 December 1986
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 67 (12), 2791-2797
- https://doi.org/10.1099/0022-1317-67-12-2791
Abstract
The RNA-dependent DNA polymerase (RDDP) of human immunodeficiency virus (HIV) was purified from sucrose density gradient-banded virus by four successive procedures: anion exchange chromatography, cation exchange chromatography, affinity chromatography on oligo(dT)-cellulose and adsorption chromatography on hydroxyapatite. The enzyme preparation was free of cellular DNA-dependent DNA polymerase activity. The properties of HIV RDDP were determined with a variety of template-primers. Generally, the enzyme used Mg2+ for optimal activity except with (Cm)n .cntdot. (dG)12-18 as template-primer. Kinetic data (Michaelis constant, Hill coefficient) were calculated for several substrates.This publication has 13 references indexed in Scilit:
- Initiation of plus-strand DNA synthesis during reverse transcription of an avian retrovirus genomeJournal of Virology, 1983
- Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for Acquired Immune Deficiency Syndrome (AIDS)Science, 1983
- A New Subtype of Human T-Cell Leukemia Virus (HTLV-II) Associated with a T-Cell Variant of Hairy Cell LeukemiaScience, 1982
- Characterization of the reverse transcriptase from a new retrovirus (HTLV) produced by a human cutaneous T-cell lymphoma cell lineVirology, 1981
- Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphomaProceedings of the National Academy of Sciences, 1980
- The isolation of avian viral RNA and polypeptides.Journal of Biological Chemistry, 1979
- DNA polymerases of normal and neoplastic mammalian cellsBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1978
- The reverse transcriptaseBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1977
- Purification and properties of Rauscher leukemia virus DNA polymerase and selective inhibition of mammalian viral reverse transcriptase by inorganic phosphate.Journal of Biological Chemistry, 1977
- HeLa cell DNA polymerase .gamma.: further purification and properties of the enzymeBiochemistry, 1976