THE USE OF METABOLITES IN THE RESTORATION OF THE VIABILITY OF HEAT AND CHEMICALLY INACTIVATED ESCHERICHIA COLI

Abstract
In the present series, the following metabolites were most effective in producing reactivation: (1) Heat "killed": Na citrate, lactic acid, and oxalacetic acid; (2) Cl "killed": Na citrate, malic acid, and oxalacetic acid; (3) H2O2 "killed": Na citrate, lactic acid, cis-aconitic acid; (4) Zephiran chloride "killed": Na citrate, lactic acid, cis-aconitic acid, and isocitric acid; (5) ethyl alcohol "killed": cis-aconitic acid, alpha-keto-glutaric acid, succinic acid, etc.; (6) the combination of 11 metabolites produced the highest reactivation.