Immunological Detection of Single Amino Acid Substitutions in Alkaline Phosphatase
- 11 April 1969
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 164 (3876), 188-189
- https://doi.org/10.1126/science.164.3876.188
Abstract
Antiserums to wild-type alkaline phosphatase from Escherichia coli were prepared and tested for reactivity with phosphatases altered by point mutations. Eight out of the nine mutant enzymes were distinguished from the wild type with quantitative microcomplement fixation. The structural changes are among the smallest yet observed immunologically.Keywords
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