Sequence analysis of the cloned streptococcal cell surface antigen I/II
Open Access
- 12 November 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 258 (1), 127-132
- https://doi.org/10.1016/0014-5793(89)81632-1
Abstract
The gene spa P (formerly designated as spa P1) encoding the M r 185,000 surface antigen (I/II) of Streptococcus mutons, serotype c (NG5), has been sequenced. The gene (4683 bp) encodes a protein of 1561 amino acid residues including putative signal peptide (residues 1–38) and transmembrane (residues 1537–1556) sequences. The N‐terminal region (60–550) has alanine‐rich repeats and is predicted to be α‐helical. However, the C‐ternunal region (800–1540) is proline‐rich and favours an extended structure. Except for a short central variable region the sequences appear to be highly conserved for S. mutans serotype c. N‐Tenninal sequencing of separated antigen I and antigen II polypeptides suggests that the former represents the N‐terminal and the latter the C‐terminal portions of the intact antigen.Keywords
This publication has 41 references indexed in Scilit:
- Homology between surface protein antigen genes ofStreptococcus sobrinusandStreptococcus mutansFEBS Letters, 1989
- Molecular characterization of a surface protein antigen gene from serotype c Streptococcus mutans, implicated in dental cariesMolecular Microbiology, 1989
- Sequence analysis of the wall‐associated protein precursor of Streptococcus mutans antigen AMolecular Microbiology, 1989
- Oligomers with Pendant Isocyanate Groups as Adhesives for Dentin and Other TissuesJournal of Dental Research, 1989
- Cloning of a surface protein antigen gene from serotype c Streptococcus mutansMolecular Microbiology, 1989
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Sequential development of helper and suppressor functions, antibody titers and functional avidities to a streptococcal antigen in rhesus monkeysEuropean Journal of Immunology, 1984
- Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteinsJournal of Molecular Biology, 1978
- Antigens in Streptococcus mutans Cross Reactive with Human Heart MuscleJournal of Dental Research, 1976
- Immunological and bacteriological basis for vaccination against dental caries in rhesus monkeysNature, 1975