Endogenous peptide(s) inhibiting [3H]cocaine binding in mouse brain
- 1 December 1980
- journal article
- research article
- Published by Springer Nature in Neurochemical Research
- Vol. 5 (12), 1291-1299
- https://doi.org/10.1007/bf00964964
Abstract
The supernatant fraction of centrifuged homogenate of brain tissue contains material that inhibits the saturable binding of [3H]cocaine to crude mouse brain membranes. This material was subjected to heat treatment to remove protein; further purification was achieved by filtering through an Amicon UM-10 membrane ultrafilter and gel filtration of the ultrafiltrate on Sephadex G-25. Sensitivity to acid hydrolysis and peptidase action indicates that the inhibitory activity resides in peptide material with a low molecular weight. The partially purified inhibitor has similar effects to that of cocaine on the specific binding of various ligands to opiate and nonopiate receptors in mouse brain membranes.This publication has 17 references indexed in Scilit:
- The pharmacological relevance of the cocaine binding site in mouse brainNeuropharmacology, 1980
- Saturable (3H)cocaine binding in central nervous system of mouseLife Sciences, 1980
- Identification of an endogenous peptide-ligand for the benzodiazepine receptorBiochemical and Biophysical Research Communications, 1980
- Dopamine receptor binding: Specificity, localization and regulation by ions and guanyl nucleotidesLife Sciences, 1978
- Cocaine: Plasma Concentrations After Intranasal Application in ManScience, 1976
- Competitive inhibition of stereospecific opiate binding by local anesthetics in mouse brainLife Sciences, 1975
- An endogenous morphine-like factor in mammalian brainLife Sciences, 1975
- Morphine-like ligand for opiate receptors in human CSFLife Sciences, 1975
- Identification of norcocaine as a metabolite of [3H]-cocaine in rat brainCellular and Molecular Life Sciences, 1974
- Compounds Affecting the Central Nervous System. 4. 3.beta.-Phenyltropane-2-carboxylic Esters and AnalogsJournal of Medicinal Chemistry, 1973