Denaturing gradient gel electrophoresis identifies genomic DNA polymorphism with high frequency in maize
- 1 July 1990
- journal article
- research article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 80 (1), 1-10
- https://doi.org/10.1007/bf00224008
Abstract
We have used denaturing gradient gel electrophoresis (DGGE) to identify genomic DNA polymorphism in maize (Zea mays L.). DGGE probes detect polymorphism in maize at a frequency comparable to the incidence of restriction fragment length polymorphism (RFLP). Probes identifying polymorphism were mapped to maize chromosome arms by utilizing DGGE and maize lines carrying B-A chromosomal translocations. The methods for library construction, probe screening, and genome analysis, described here for maize, can also be applied to the genomic analysis of other organisms.Keywords
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