A radioimmunoassay for abscisic acid
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 146 (2), 139-145
- https://doi.org/10.1007/bf00388224
Abstract
We have developed a radioimmunoassay (RIA) for abscisic acid (ABA) in the 0.1 ng to 2.5 ng range. Antibodies were obtained from rabbits immunized with ABA bound via its carboxyl group to bovine serum albumin. Cross-reactivity studies indicate that ABA esters are completely cross-reactive with ABA, while trans, trans abscisic acid (t-ABA) phaseic acid (PA) and dihydrophaseic acid (DPA) have much lower but significant cross-reactivities. Purification methods which reduce the levels of cross-reacting substances are described.This publication has 22 references indexed in Scilit:
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