The effect of nociceptin on Ca2+ channel current and intracellular Ca2+ in the SH‐SY5Y human neuroblastoma cell line
- 1 May 1996
- journal article
- Published by Wiley in British Journal of Pharmacology
- Vol. 118 (2), 205-207
- https://doi.org/10.1111/j.1476-5381.1996.tb15387.x
Abstract
The human neuroblastoma cell line SH-SY5Y expresses the ‘orphan’ opioid receptor (ORL1). We have demonstrated that nociceptin, the putative endogenous ligand for ORL1, produces a concentration-dependent inhibition of the N-type calcium channel current in these cells (IC50 42 nM). In addition, in the presence of carbachol, nociceptin increased the intracellular concentration of Ca2+ (EC50 60 nM). Both effects of nociceptin were blocked by pertussis toxin pretreatment but not by the opioid antagonists CTAP (1 μm), naltrindole (1 μm) and naloxone (10 μm).Keywords
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