The identification, characterization, and mapping of a gene for flocculation in Saccharomyces sp.

Abstract

Genetic studies of a flocculent haploid strain of Saccharomyces revealed the flocculation to be dominant and controlled at a single gene locus. The flocculation character of hybrids and haploids derived from such hybrids appears to be influenced by the repression or derepression status of the culture. Mapping studies of this flocculation gene revealed that it is linked to ade 1 and therefore located on Chromosome I. This is a different gene to the 3 flocculation genes studied by other laboratories because they found such genes to be unlinked to ade 1. The flocculation gene being discussed in this paper was designated FLO4. FLO4 was located 32-33 cM from the Chromosome I centromere and 37 cM from ade 1 (i.e., FLO4 is on the opposite side of the centromere to ade 1).