Attachment of Motile Bacterial Cells to Prealigned Holed Microarrays
- 8 November 2006
- journal article
- Published by American Chemical Society (ACS) in Langmuir
- Vol. 22 (26), 11251-11254
- https://doi.org/10.1021/la0609726
Abstract
Construction of biomotors is an exciting area of scientific research that holds great promise for the development of new technologies with broad potential applications in areas such as the energy industry and medicine. Herein, we demonstrate the fabrication of prealigned microarrays of motile Escherichia coli bacterial cells on SiOx substrates. To prepare these arrays, holed surfaces with a gold layer on the bottom of the holes were utilized. The attachment of bacteria to the holes was achieved via nonspecific interactions using poly-l-lysine hydrobromide (PLL). Our data suggest that a single motile bacterial cell can be selectively attached to an individual hole on a surface and bacterial cell binding can be controlled by altering the pH, with the greatest occupancy occurring at pH 7.8. Cells attached to hole arrays remained motile for at least 4 h. These data indicate that holed surface structures provide a promising footprint for the attachment of motile bacterial cells to form high-density site-specific functional bacterial microarrays.Keywords
This publication has 6 references indexed in Scilit:
- Role of Lipopolysaccharides in the Adhesion, Retention, and Transport of Escherichia coli JM109Environmental Science & Technology, 2003
- Antibody-based immobilization of bioluminescent bacterial sensor cellsTalanta, 2001
- Self-Assembled Monolayers That Resist the Adsorption of Proteins and the Adhesion of Bacterial and Mammalian CellsLangmuir, 2001
- Measuring the forces involved in polyvalent adhesion of uropathogenic Escherichia coli to mannose-presenting surfacesProceedings of the National Academy of Sciences, 2000
- Polystyrene, Poly-L-Lysine and Nylon as Adsorptive Surfaces for the Binding of Whole Cells ofMycobacterium TuberculosisH37 RV to Elisa PlatesJournal of Immunoassay, 1990
- Pausing, switching and speed fluctuation of the bacterial flagellar motor and their relation to motility and chemotaxisJournal of Molecular Biology, 1990